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0 and 45 °C exhibited that each one three protease preparations could arrive at a maximal DH in eight h under the disorders made use of. Alcalase hydrolysates displayed the most beneficial antioxidant Houses in DPPH, ABTS, and FRAP antioxidant routines. Concurrently, underneath the exact same disorders, the DH and antioxidant Homes of The 2 types of roe proteins ended up decreased While using the inclusion of defatting and freeze-drying treatments, likely as a consequence of variations in possibly protein morphology or loss of antioxidant peptides through lipid extraction, and this involves further more analysis.
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The diploma of hydrolysis from the samples was resolute according to the L-serine equivalent system. The information were being obtained from 3 unbiased hydrolyses for every roe and protease combination. Examination of variance (ANOVA) was performed. Letters a–c reveal major differences amid unique samples prepared with varying enzyme concentrations simultaneously position (p < 0.05).
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